Means for introducing microbe cultures into bottles or other vessels.



1?. N. E. REMY.

MEANS FOR INTRODUCING MIGROBE CULTURES INTO BOTTLES OR OTHER VESSELS.APPLIOATION FILED DEO.19, 190'7.

988,031. Patented Max 28, 1911..

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B AT PT PIERRE NICOLAS EMILE REMY, OF PARIS, FRANCE, AfiSIGNOR T0SOCIE'IE LE FERMENT, OF PAR-IS, FRANCE.

MEANS FOR INTRODUCING MICROBE CULTURES INTO BOTTLES OR OTHER VESSELS.

Specification 01' Letters Patent.

Patented Mar. 28, 19151.

Application filed December 19, 1907. Serial No. 407,169.

To all whom it may concern;

Be it known that I, PIERRE NrooLAs EMILE REMY, a citizen of theRepublicof France, residing at Paris, France, have mvented certain newand useful Improvements in Means for Introducing Microbe Cultures intoBottles or other Vessels, of which the following is a specification.

This invention relates to means whereby at any moment a vesselcontaining a sterilized broth or liquid can be inoculated with a microbeculture and it consists in lntroducing into the neck of the bottle orother vessel a tube containing the microbe culture the flange of whichtube rests upon the mouth of the vessel; a cover forms a hermeticclosing for the inoculating tube, and by introducing a metal rod througha hole made through the center of the cover a plug in the bottom of thesaid tube can be forced out and the culture caused to fall into thesterilized broth or liquid.

To enable the invention to be fully understood I will describe it withreference to the accompanying drawing in which:

Figure 1 is an elevation and Fig. 2 a vertical section of the neck of abottle provided with an inoculating tube according to the invention.

In the neck of the bottle is introduced a tube B of metal, preferablyaluminium, which contains the liquid or dry microbe culture. This tubeis made with a flaring edge a which rests on the upper part of the neckand a washer b of india-rubber or other elastic material is interposedbetween the flaring edge of the tube B and the mouth of the bottle. Thetube B is provided at its lower part with a plug or stopper 0 whichcloses it hermetically; this plug 0 is advantageously connected to thelower part of the tube B by a circular ligature (Z of metallic wire anda metal wire 6 which extends to the said plug and forms a hinge.

The bottle is provided with a cover C of glass, porcelain or othermaterial which closes it hermetically and is similar to the stopperswhich are sometimes used for closing beer bottles, the said cover beingprovided with an india-rubber washer 0. It is hinged to a metal collar fby means of alink 9 and the fastening of the cover upon the bottle, orrather upon the flaring edge of the metal tube B is eflected by means ofa stirrup h which is acted on by an eccentric portion 71:. Just as thelink 9 is hinged at one end of the cover C the securing stirrup it actsat the other end in a suitable notch m in the cover. The cover isprovided in its center with a hole which receives an indiarubber plug itwhich closes it hermetically.

The bottle being filled with sterilized liquid and the tube B whichcontains the microbe culture being placed in the neck, as shown in thedrawing, the sterilized liquid can remain in this state as long asrequired. In order to inoculate the sterilized liquid it is onlynecessary to remove the small plug a from the cover and to force theplug 0 out of the tube B by means of a metal or other rod introducedinto the extended central hole in the cover C. The microbe culturecontained in the tube B will then fall into the sterilized broth orliquid contained in the bottle and multiply therein.

Having now particularly described and ascertained the nature of my saidinvention and in what manner the same is to be performed, I declare thatwhat I claim is:

1. Means for inoculating sterilized broths or liquids with microbecultures, including in combination a vessel for containing the broth orliquid, a tube for containing the microbe cultures adapted to beremovably introduced into the neck of said vessel and having at itsupper part a flaring edge adapted to rest upon the mouth of the vessel,a plug closing the lower part of said tube, a cover adapted to rest uponthe flaring edge of the tube, a washer interposed between the mouth ofthe vessel and the edge of the tube, a second washer introduced betweenthe edge of the tube and the cover, said cover having a hole through it,and a hermetic plug adapted to close the hole in said cover and adaptedto be removed to permit the introduction of a device for unstopping thebottom of the tube so as to permit the microbe cultures to fall into thebroth.

Means for inoculating a sterilized broth or liquid with microbecultures, including in combination a vessel adapted to contain thebroth,a tube B adapted to be removably in troduced into the neck of saidvessel and to contain the microbe cultures and having at its upper parta flaring edge (4 adapted to rest upon the mouth of the vessel, a plug 0closing the lower part of said tube, a circular ligature (Z and a wire eforming a hinged fastening of said plug to said tube a heruurtic cover Ccarrying a Washer 0 and adapted to lit upon the flared edge of the tube,a Washer b interposed between the flared edge of the tube and the mouthof the vessel, the said cover hating a hole therethrough, and a plug aclosing said hole in the cover and removable to permit the introductionof a rod for pushing out the plug 0 and permitting the introduction ofthe microbe cultures into the liquid.

Means for ineculating a sterilized broth or liquid with microbe culturesincluding in combination a vessel adapted to contain the broth, a tubel3 adapted to be reinovably introduced into the neck of said vessel andto contain the microbe cultures and having at its upper part a flaringedge a ada ted to rest upon the mouth of the vessel, a plug 0 closingthe lower part of said. tube, a circular ligature (Z and a Wire eforming a hinged fastening of said plug to said tube a herinet-ic coverC carrying a Washer 0 and adapted to fit upon the flared edge of thetube, a Washer 6 interposed between the flared edge 01' tne tube and themouth of the vessel, the said cover having a hole therethrough, and aplug a closing said hole in the cover and removable to permit the introduction oi a rod for pushing out the plug 0 and permitting theintroduction of the inicrobe cultures into the liquid, a metal collar fand a link 9 by Which the cover is hinged and connected to said collar,a stirrup 7L adapted to engage the cover at a point opposite the hingedside and an. eccentric device for pulling down said stirrup or releasingit.

In Witness whereof I have hereunto set my hand in presence of twoWitnesses.

PlERRE NICOLAS EMILE REMY. llitnesses:

Juries jiminxmt'o, Jeune DEAN lt-LisoN.

Copies of this patent may be obtained for five cents each, by addressingthe Commissioner of Patents, Washington, 13. C.

